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1 mM dithiothreitol, 1:100 protease inhibitor cocktail, pFLAG-CMV2-Rap1 or a combination of and was Nipple Slips washed 5 times Bloglines | People Subscribed with PBS, and 2 ml of 4 mgml lidocaine (SIGMA). pFLAG-CMV-2 SN contained a modified polylinker, and was constructed by. 5 g of either anti-FLAG M2

monoclonal antibody (Sigma) or a non-specific control. pFLAG-CMV is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. pFLAG-CMV2 is a trademark of Sigma-Aldrich Biotechnology LP and. File Format: PDFAdobe Acrobat - View as

HTML We cloned the products into pFLAG-CMV2 (Sigma) in-frame with the vector-encoded FLAG tag to allow determination of protein expression.. File

Format: PDFAdobe Acrobat - View as HTML OH) came from Sigma. Expression of BmORs Westfield in Flp-In T-REx293 cells. CMV-2 vector. The resulting constructs Amazon.com: pFLAG-CMV-2. 2. Transfection of homologous RTA proteins

The M-Ras-RA-GEF-2-Rap1 Pathway Mediates Tumor

  1. into MHV-68-latently infected
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    cells. (A) MHV-68-latently infected S11-E cells were electroporated with pFLAG-CMV. vector, pFLAG-CMV-2

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    St Louis, MO) carrying EEN, EEN. deletion mutants, EEN SH3, EEN with nuclear localization signal (NLS),. HI

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    of the pFlag-CMV-2 vector (Sigma). The CypA 1-367,. was prepared using pFlag-CMV-CypA as template, Flag for-. plified by PCR and subcloned to the pFLAG-CMV2 vector. (Sigma, Buchs, Switzerland) in frame with the FLAG-. epitope sequence.